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Firstly, temperature affects the working of enzymes.Enzymes are destroyed by high temperatures,the proteins in the enzymes become denatured as they lose their specific shape.The process of digestion of food cannot be carried out anymore.In fact, each enzyme has an optimum working temperature.

Secondly,the acidity and alkalinity of the environment where the enzymes work does affect the working of enzymes too.Different enzymes work best under different pH valued environments.Most enzymes work best at pH value 7.Others then work best in acidic or alkaline mediums.Enzymes too become denatured if the pH value of the medium is incorrect.